The E. coli vtx1 and vtx2 Subtyping PCR Kit can detect all vtx subtypes as well as the eae gene.
The E. coli vtx1 and vtx2 Subtyping PCR Kit contains seven primer mixes, seven PCR positive DNA controls, PCR ReadyMix (including loading buffer), TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8) and 10% Chelex 100 in TE buffer (magnetic stir bar added). In addition, a test panel of two samples is included in order to test the temperature calibration of the in-house thermal cycler.
Each primer mix is marked with a coloured lid. The same colour is used in the packaging insert to mark the procedure and data for each primer mix.
The kit contains enough PCR ReadyMix to perform approximately 200 PCR reactions. Primer mix vtx/eae Detection is intended for screening, and the vial contains reagent enough for 100 reactions. Primer mixesvtx1 Subtyping, vtx2a/e Subtyping, vtx2c Subtyping and vtx2dSubtyping contain reagents enough for 70 reactions each, whereas primer mix vtx2b/f Subtyping and vtx2g Subtyping contain reagents enough for 45 reactions. The volumes of the primer mixes are adjusted according to the frequency of subtypes found. Each control DNA vial contains 150 μL, corresponding to at least 25 tests.
The E. coli vtx1 and vtx2 Subtyping PCR Kit is targeted for in vitrodiagnostic PCR subtyping of the verocytotoxin encoding E. coli genesvtx1 and vtx2. In addition the vtx/eae Detection primer mix detects if the vtx type combines with the eae gene encoding intimin.
The recovery (percentage of samples giving the expected result) was between 97% and 100% when compared with results obtained by doing PCR, serotyping, and/or DNA hybridisation technique of The International Eschericia and Klebsiella Centre (WHO).
| Primer Mix | Recovery % |
|---|---|
| vtx/eae Detection | 97 % |
| vtx1 Subtyping | 100 % |
| vtx2a/e Subtyping | 98 % |
| vtx2b/f Subtyping | 100 % |
| vtx2c Subtyping | 98 % |
| vtx2d Subtyping | 98 % |
| vtx2g Subtyping | 100 % |
| Article number | Product | Number of tests | Packing |
|---|---|---|---|
| 91346 | E. coli vtx1 and vtx2Subtyping PCR Kit | 200 |
1 box |
