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LS28   Optima
•Standard PCR
•RT-PCR
•Very complex templates
•GC-rich templates
•SNP-typing
•Multiplex PCR
•Many others...

商品功能說明

The optimum of both polymerase families 

The FastGene® Optima is a mixture of a highly purified Taq Polymerase and a modified type-B polymerase with proof-reading  abilities. The enzymes are purified using three different chromatography technologies and result in a very high purity and very high activity. The FastGene® Optima is extremly robust and was developed for the standard PCR, difficult as well as very long amplicons of over 7.5 kBp. 

 

Optima(l) robustness for very complex samples

The FastGene® Optima can handle very complicated templates. The highly purified Taq polymerase gives high efficiency while the proof-reading polymerase guarantees the fidelity. The robustness of both enzymes makes the amplification of complex tissue, such as liver (Fig. 1), possible.

 

Fig. 2: Comparing the ability of Competitor T‘s and  FastGene® Optima polymerase mixture to amplify GC-Rich DNA fragments. Two fragments of 60.7 % and 64.3% were amplified resulting in two products of 1839 bp and 1260 bp, repectively. FastGene® Optima had a higher efficiency compared to Competitor T‘s polymerase mixture. 
Data was kindly provided by Ms. Ryoko Nakayama, Department of Pathology, Tsurumi University, Japan.

Optima(l) for SNP-typing

The detection of single nucleotide polymorphism (SNP) requires extreme fidelity. The proof-reading activity guarantees this needed fidelity (see Fig.3).

 

Fig. 3: SNP typing of the ALDH gene using FastGene® Optima polymerase. The ALDH classified as human sensitivity to alcohol gene was analysed for presence of a SNP by digesting the amplification of homo- and heterozygotes using MboII.
Data was kindly provided by Dr. Che Xiano-Fang, Department of Biochemistry, Tokyo Medical University, Japan.

 

 

Cat. No. Product Content
LS28 FastGene® Optima PCR kit  250 Units