The Dermatophyte PCR Kit is a multiplex PCR system that enables determination of dermatophytes generally and T. rubrum from nail samples within 5 hours.
Nail infections are caused chiefly by T. rubrum and T. mentagrophytes. Traditionally, these species are identified from nail samples by culturing on medium, which takes 10-15 days to 3-4 weeks. The Dermatophyte PCR Kit is a multiplex PCR system that enables determination of dermatophytes generally and T. rubrum from nail samples within 5 hours. The primer mix contains two primer pairs aimed at, respectively, chitin synthase 1 for detection of dermatophytes generally and ITS2 (internal transcribed spacer) to detect T. rubrum. Furthermore, the primer mix contains an internal plasmid control that functions as a template for the primer pair specifically for T. rubrum.
By comparing the dermatophyte multiplex PCR method with conventional methods of identifying nail infections, it has been shown that the PCR method identifies most dermatophyte and T. rubrum-positive nail samples (ref. 1, 2, 3 and 4).
The kit contains all reagents necessary to carry out dermatophyte PCR reactions.
The kit contains Buffer A and Buffer B to prepare the template, primer mix and PCR ReadyMix (including loading buffer) to run the samples and two PCR-positive DNA controls—one containing dermatophyte genomic DNA and one containing T. rubrum genomic DNA.
There is enough reagent for 100 PCR reactions in the kit.
The Dermatophyte PCR Kit is used for in vitro diagnostics of dermatophytes in general and T. rubrum from nail samples.
Article number |
Product |
Number of tests |
Packing |
---|---|---|---|
73411 |
Dermatophyte PCR-kit |
100 |
1 box |